Studying (a) binary fission in Amoeba, and (b) budding in yeast and Hydra with the help of prepared slides.
CBSE Class 10 Practicals Science Preparing a temporary mount of a leaf peel to show stomata. Studying (a) binary fission in Amoeba, and (b) budding in yeast and Hydra with the help of prepared slides. Students get here clear concept at this page.
Finding stomata | CBSE Class 10 Practicals | Binary fission in Amoeba Practicals | Class 10 Science Practicals | Class 10 Projects | Budding in Yeast Projects | Focal Budding in Hydra Practicals
Preparing a temporary mount of a leaf peel to show stomata.
Studying (a) Binary fission in amoeba (b) Budding in yeast and Hydra with the help of prepared slides.
Aim: Preparation of a temporary mount of a leaf peel to show stomata.
Requirements: A leaf of plant, needle, watch glass, coverslip, safranine, dropper, glycerin and compound microscope.
Procedure:
1) Take a fresh leaf from healthy and non diseased plant.
2) Peel the leaf and pull a lower section of a leaf with the help of forceps.
3) Keep the leaf peels in watch glass containing water.
4) Transfer the peel into another watch glass, with the help of dropper add some drops of safranine in the watch glass.
5) Take a clean glass slide and transfer the leaf peel to glass slide after 2-3 minutes.
6) Add a drop of glycerin and put cover slip on the glass slide.
7) Gently press the cover slip to set the glass slide.
8) If excess glycerin is present, remove it with the help of blotting paper.
9) Observe the slide under microscope.
Observation:
1) Nucleus is present in each stomata.
2) Stomata are enclosed by the guard cells.
3) Stomata contains chloroplast and vacuole.
4) Guard cells protected by cell wall.
Result: opening and closing of stomata were observed.
Conclusion: Stomata from leaf peel were successfully observed.
(a) Binary fission in amoeba, Budding in yeast and Hydra.
Aim: To study binary fission in amoeba with the help of prepared slides and Budding in yeast and Hydra.
Requirements: Prepared slide of binary fission in amoeba, budding in yeast and hydra and compound microscope.
Procedure:
1) Take a prepared slides labeled with amoeba in binary fission, budding in yeast and hydra.
2) Adjust the slide under microscope first with low power objective and then high power objective.
3) Record the observation and draw the diagram.
Observation:
(a) Binary fission in amoeba
1) Binary fission in amoeba is a type of asexual reproduction.
2) In which within the slide, from mother amoeba, 2 daughter amoeba cells were observed.
3) Presence of pseudopodia is necessary for binary fission.
(b) Budding in yeast and Hydra
1) In yeast and hydra from the parent cell, the outgrowth emerges and this outgrowth leads to formation of new organism.
2) In the slide, budding was observe in yeast and hydra.
Budding in yeast and hydra
Result: From the prepared slides, successful observation of binary fission in amoeba and budding in yeast and hydra done.
Conclusion: From the slides, it was concluded that binary fission occurs in amoeba and budding in yeast and hydra.
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